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Questions

  1. True or false: Köhler illumination produces flat illumination, i.e. the light intensity does not vary across the sample.

  2. Assume you would like to take a time series acquisition of live cells. You use Micro-Manager and configure a Multi-Dimensional Acquisition with 50 ms exposure time, 10 frames, and 0 time interval between frames. True or false: the total acquisition time is 500 ms. (Hint: what is the readout time for the Photometrics Prime camera?)

  3. True or false: the value of a pixel depends only on the rate of photons hitting it.

  4. Why do we avoid saturating the pixels when imaging? (A pixel is saturated when its value is equal to its maximum value. For a 16 bit camera, this value is 2^16 - 1 = 65535.)