1. 1. Introduction
2. 1. 1.1. Learning Outcomes
3. 2. Microscope Optics and Image Acquisition
4. 1. 2.1. The Epifluorescence Microscope
   2. 2.2. Set up the Camera
   3. 2.3. Align the Camera and Tube Lens
   4. 2.4. Set up Micro-Manager
   5. 2.5. Discussion
   6. 2.6. Questions
5. 3. Alignment
6. 1. 3.1. Construct the Microscope Emission Path
   2. 3.2. Control the Laser
   3. 3.3. Construct the Microscope Excitation Path
   4. 3.4. Discussion