Questions

1. What is the difference between an excitation filter and a dichroic mirror?

2. Why do we use an emission filter in addition to a dichroic mirror in the fluorescence light path?

3. True or false: fluorescence light has a shorter wavelength than that of the excitation light.

4. What are the differences between industrial CMOS and scientific CMOS (sCMOS) cameras?

5. True or false: A camera with an exposure time of 20 ms necessarily acquires frames at a rate of 50 frames per second.

6. Does the distance between the objective and the tube lens matter? Why or why not?

7. Why do we do fluorescence imaging with epi-illumination and not transillumination?